Long R3 IGF-1 premixed?

[DarkVader]

Exactly how long does this stuff last once it's mixed into solution? for eg. how long would the solution that muscle research carries last? How should i store it?

[SMILEY FACE]

never used it sorry

[JohnnyB]

Long™R3IGF-I
Recombinant analog of human insulin growth factor-I

Description

Long™R3IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) that has been specifically engineered for the enhancement of cell culture performance. Long™R3IGF-I is more biologically potent in vitro than either insulin or native IGF-I and has been documented to
significantly increase recombinant protein production. It is ideal for both research and large-scale culture systems utilizing serum-free or low-level serum applications. Long™R3IGF-I is effective in any cell that contains type I IGF receptors, including most Chinese Hamster Ovary (CHO) lines, hybridomas and fibroblasts. Long™R3IGF-I is produced in a patented E. coli expression system without the use of animal-derived components.

Precautions

This product is for research/laboratory use. THIS PRODUCT
IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.

Storage

Long™R3IGF-I in lyophilized form should be stored at 2 to 8 C and is stable for at least 3 years. Liquid solutions of Long™R3IGF-I (prepared as described below) can be stored
at 2 to 8 C and are stable for at least 2 years.

Preparation Instructions

1. Long™R3IGF-I is supplied lyophilized in an atmosphere of nitrogen at a slight vacuum (-25 kPa). Remove the metal cap from the glass vial and introduce an air filled syringe
through the septum to equalize the pressure.

2. Add sufficient 10 mM HCl or 100 mM acetic acid solution to the vial to achieve a peptide concentration of at least 0.1 mg/mL. Concentrations of 1 mg/mL or more are recommended.

3. Mix the solution thoroughly to insure the peptide is completely dissolved.

4. Filtration of a Long™R3IGF-I solution or a culture medium after the addition of Long™R3IGF-I should be performed using a low protein-binding membrane such as Polyvinyl Idene Flouride (PVDF), Poly Ethylene Sulfite (PES) or Cellulose Acetate (CA).

Methods for Use

Once Long™R3IGF-I has been reconstituted, it can then be added directly to cell culture medium. A titration of Long™R3IGF-I should be performed for each different application as the optimum concentration may vary depending upon the cell type used and other components present in the medium. The recommended final concentration range of Long™R3IGF-I is 10 to 50 µg/L (or ng/mL). Because Long™R3IGF-I and insulin may compete for the same cell receptors, the effectiveness of Long™R3IGF-I will be masked if it is added in conjunction with commonly employed concentrations of insulin (1 to 10 mg/L). However, inclusion of physiological levels of insulin (5 to 10 µg/L) in cell culture medium containing the recommended levels of
Long™R3IGF-I results in beneficial synergistic effects in certain applications.

Characteristics

Appearance
White powder

Molecular weight
9110 ± 2 daltons (Mass spectroscopy)

Endotoxin
< 0.1 EU/µg

Biological Activity
ED50 < 10 ng/mL (Bioassay assessing the stimulation of
protein synthesis in L6 myoblasts)

Identity/Consistency
Confirmed by N-terminal sequence analysis and HPLC

References
1. Francis, G., et al. J. Mol. Endocrinol. (1992) 8:213.
2. Thomas, J., Fung, V. Animal Cell Technology: Products of
Today, Prospects for Tomorrow (1993) 91.
3. Morris, A., Schmid, J. Biotechnol. Prog. (2000) 16:693.

JohnnyB

[JohnnyB]

2C=36 degrees 8C=46 degrees, so if it's refigerated 2 years. I keep mine in the freezer.

JohnnyB

[DarkVader]

Ok i my Long™R3IGF-I today it came premixed in a 1mg clear solution. I added 3ml of what i though was bacteriostatic water but was bacteiostatic sodium chloride instead(shipping error). Now my Long™R3IGF-I is cloudy. Is it still good? will it last the same amount of time?

[AsianDynamo]

ok so could i get premix long r3 igf-1 delivered to the uk and would it be ok, becuase i've read new threads that say when kept with alcohol it will be ok for alot longer and might be able to survive shipping?

[AsianDynamo]

bump, come on guys help a guy out, it's impossible to get in the uk and if you do it costs more than hgh.

[JsJs24]

bump, come on guys help a guy out, it's impossible to get in the uk and if you do it costs more than hgh.

You do not pre mix igf-1 because it will ruin it. I pull back some water (about 30 units) and then the igf-1 and shoot it immediately.

[AsianDynamo]

"Storage

Long™R3IGF-I in lyophilized form should be stored at 2 to 8 C and is stable for at least 3 years. Liquid solutions of Long™R3IGF-I (prepared as described below) can be stored
at 2 to 8 C and are stable for at least 2 years."

so doesn't that mean you can pre mix it and store it without ruining it?

so should i order it in powder form and would it be ok when i get it in the uk?

[JsJs24]

"Storage

Long™R3IGF-I in lyophilized form should be stored at 2 to 8 C and is stable for at least 3 years. Liquid solutions of Long™R3IGF-I (prepared as described below) can be stored
at 2 to 8 C and are stable for at least 2 years."

so doesn't that mean you can pre mix it and store it without ruining it?

so should i order it in powder form and would it be ok when i get it in the uk?

I have never heard of premixing it. Mine comes already in liquid form but potent (1mg/ml). Now we are talking about pulling back about 3 or 4 units of this stuff so bact. water is necessary to ensure you get it all out and to help it spread inside your body as well. Do not mix bact. water with this! I was told by the company that makes mine that it will ruin it for certain.

If you get igf in powder form then I don't know what to tell you, mine is already mixed.