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Anti-Human Myostatin Bovine Polyclonal Antibody,
Immunoaffinity Purified
Cat. No.: RD181**522*
Introduction:
Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor ß superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.
Specification: Anti-Human Myostatin Antibody was raised in bovine against Human Myostatin His-Tagged Fusion Protein.
Specificity: The recombinant human myostatin is 100% homologous with the human serum myostatin.
Source of Antigen: E. coli
Host: Bovine
Purification Method: Immunoaffinity chromatography on a column with immobilized human myostatin. Endotoxin free.
Antibody Content: 0.1 mg (determined by UV spectroscopy method, 280nm)
Titer: ELISA: >1:50,000 for antibody concentration 1 mg/ml
Formulation: Sterile filtered and lyophilized from 2 mg/ml in 0.2 M phosphate buffer, 0.2 M NaCl, pH 7.2
Reconstitution: Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage: Store lyophilized antibody at 2-8°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Stability/Shelf Life: The lyophilized antibody remains stable and fully active until the expiry date when stored at 4°C.
Quality Control Test: ELISA - to determine titer of the antibody
Applications: ELISA, Western blotting
Note: The Anti-Human Myostatin Bovine Polyclonal Antibody is for research use only.
References:
McPherron A.C. and Lee S.J.: Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA 94, 12457-12461 (1997)
Sharma M. et al.: Myostatin, a transforming growth factor ß superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. Journal of Cellular Physiology 180:1-9 (1999)
Gonzalez-Cadavid N.F., Taylor W.E. et al.: Organization of the human myostatin gene and expression in heathy men and HIV-infected men with muscle wasting. Proc. Natl. Acad. Sci. USA 95, 14938-14943 (1998)
Immunoaffinity Purified
Cat. No.: RD181**522*
Introduction:
Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor ß superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.
Specification: Anti-Human Myostatin Antibody was raised in bovine against Human Myostatin His-Tagged Fusion Protein.
Specificity: The recombinant human myostatin is 100% homologous with the human serum myostatin.
Source of Antigen: E. coli
Host: Bovine
Purification Method: Immunoaffinity chromatography on a column with immobilized human myostatin. Endotoxin free.
Antibody Content: 0.1 mg (determined by UV spectroscopy method, 280nm)
Titer: ELISA: >1:50,000 for antibody concentration 1 mg/ml
Formulation: Sterile filtered and lyophilized from 2 mg/ml in 0.2 M phosphate buffer, 0.2 M NaCl, pH 7.2
Reconstitution: Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage: Store lyophilized antibody at 2-8°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Stability/Shelf Life: The lyophilized antibody remains stable and fully active until the expiry date when stored at 4°C.
Quality Control Test: ELISA - to determine titer of the antibody
Applications: ELISA, Western blotting
Note: The Anti-Human Myostatin Bovine Polyclonal Antibody is for research use only.
References:
McPherron A.C. and Lee S.J.: Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA 94, 12457-12461 (1997)
Sharma M. et al.: Myostatin, a transforming growth factor ß superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. Journal of Cellular Physiology 180:1-9 (1999)
Gonzalez-Cadavid N.F., Taylor W.E. et al.: Organization of the human myostatin gene and expression in heathy men and HIV-infected men with muscle wasting. Proc. Natl. Acad. Sci. USA 95, 14938-14943 (1998)
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