Is this what I think it is?

[Piason]

TweetAnti-Human Myostatin Bovine Polyclonal Antibody,
Immunoaffinity Purified


Cat. No.: RD181**522*
Introduction:
Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor ß superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.

Specification: Anti-Human Myostatin Antibody was raised in bovine against Human Myostatin His-Tagged Fusion Protein.

Specificity: The recombinant human myostatin is 100% homologous with the human serum myostatin.

Source of Antigen: E. coli

Host: Bovine

Purification Method: Immunoaffinity chromatography on a column with immobilized human myostatin. Endotoxin free.

Antibody Content: 0.1 mg (determined by UV spectroscopy method, 280nm)

Titer: ELISA: >1:50,000 for antibody concentration 1 mg/ml

Formulation: Sterile filtered and lyophilized from 2 mg/ml in 0.2 M phosphate buffer, 0.2 M NaCl, pH 7.2

Reconstitution: Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Storage: Store lyophilized antibody at 2-8°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.

Stability/Shelf Life: The lyophilized antibody remains stable and fully active until the expiry date when stored at 4°C.
Quality Control Test: ELISA - to determine titer of the antibody

Applications: ELISA, Western blotting

Note: The Anti-Human Myostatin Bovine Polyclonal Antibody is for research use only.

References:
McPherron A.C. and Lee S.J.: Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA 94, 12457-12461 (1997)

Sharma M. et al.: Myostatin, a transforming growth factor ß superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. Journal of Cellular Physiology 180:1-9 (1999)

Gonzalez-Cadavid N.F., Taylor W.E. et al.: Organization of the human myostatin gene and expression in heathy men and HIV-infected men with muscle wasting. Proc. Natl. Acad. Sci. USA 95, 14938-14943 (1998)

[flexer01]

TweetGH?

[jack hust]

Tweetcant tell what it is bro

[Piason]

TweetIt kind of sounds like a human myostatin blocker??

I guess if nobody has any idea I will have to e-mail the manufacturer..

Thanks,
Piason.......

[chicken hawk]

Tweetwhat up dude,

Western blotting and ELISA are test methods that we microbiologists(yes I am!!!) use to determine a number of different things about antigens and antibodies. This sounds very legit. Wouldn't happen to be made by Sigma Aldrich would it?

[chicken hawk]

TweetIt is an antibody that "knocks out" human myostatin. It is made by injecting cows with human myostatin and the cows make antibodies against this myostatin because it is a foreign substance in their bodies. It is sterile...it is also lyophilized(freeze dried) so you have to reconstitute with water. After recon. you need to put in sepirate vials and put in the freezer.

[Piason]

TweetChicken hawk

So you think that this sounds like a human myostatin blocker,that is very exciting.

Have you known of a working myostatin blocker prior to this info?

This was not made by Sigma Aldrich.

Thanks,

Piason.......

[Piason]

TweetChicken hawk

Below is another product they make, it sounds similar to the one above (for the average joe), What are the diferances? if any.

This one is also more expensive then the one above.



Human Myostatin His-Tagged Fusion Protein


Cat. No.: RD172**53**

Introduction:
Myostatin (GDF-8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor â superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.

Description:
The Human Myostatin is created as a recombinant protein with N-terminal fusion of His-Tag with aded 31 amino acid sequence as a spacer (Met-Arg-Gly-Ser-His-His-His-His-His-His-Gly-Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly-Arg-Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys-Asp-Pro-Ser-Ser-Arg-Ser-MYO). The Human Myostatin His-Tagged Fusion Protein, produced in E. coli, is 16 kDa protein containing 113 amino acid residues of the human myostatin and 37 additional amino acid residues.

Specificity: The human myostatin part of the fusion protein is corresponding to amino acid sequence of the human myostatin (Acc. No. AF019627, R263-S376).

Purification Method: Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding

Protein Content: 0.1 mg (determined by BCA method)

Purity: Purity of Human Myostatin His-Tagged Fusion Protein is>95% (SDS-PAGE analyzed).

Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 0.1 M acetate buffer, pH 5.5

Reconstitution: Add 0.1 ml of 0.05 M acetate buffer pH 4 and let the lyophilized pellet dissolve completely.

Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Stability/Shelf Life: The lyophilized protein remains stable for at least 1 year when stored at 4°C (and reconstituted for at least 1 year when stored at -20°C).

Quality Control Test: BCA - to determine content of the protein
SDS-PAGE - to determine purity of the protein

Applications: Western blotting, ELISA

Note: The Human Myostatin His-Tagged Fusion Protein is for research use only.

References:
McPherron A.C. and Lee S.J.: Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA 94, 12457-12461 (1997)

Sharma M. et al.: Myostatin, a transforming growth factor â superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. Journal of Cellular Physiology 180:1-9 (1999)

Gonzalez-Cadavid N.F., Taylor W.E. et al.: Organization of the human myostatin gene and expression in heathy men and HIV-infected men with muscle wasting. Proc. Natl. Acad. Sci. USA 95, 14938-14943 (1998)

[chicken hawk]

TweetThis is the actual myostatin protein...you don't want this...you are trying to inactivate myostatin because it puts a "cap" on muscle growth. In theory if you inactivate myostatin then the muscles could grow much larger than genetically possible because their growth wouldn't be regulated.

A bacteriostat stops bacterial from growing/reproducing so a myostat would stop/hinder muscle growth. Make sense?

The Hawk

[chicken hawk]

TweetDude you want to ask the manufacturer if the anto-myostatin antibody will actually inactivate human myostatin or if it is used simply bind with myostatin for identification asseys.

[flexer01]

Tweetlol......nice thread.

[Piason]

TweetI just sent an e-mail to the manufacturer asking this:

Does your Anti-myostatin antibody actually inactivate human myostatin, or is it used simply to bind with myostatin for identification asseys.

I will report back as soon as I get a reply...

Piason......

[chicken hawk]

Tweetgood deal bro

[trancedelt]

Tweetwhen someone comes out with a site-specific myostatin blocker let me know...

i dont like the idea of my face and my heart bulking up

[Piason]

TweetHere is what they wrote back:

Dear Mr. *******,

Thank you for your email and interest in our product. I am afraid, we don't
know if our anti-myostatin antibody inactivate human myostatin. Are you a
researcher who is working in this field? Can you measure such interactions?

Best regards,

Mgr. ***** *******,
Sales Manager & Technical Support


What now, do they not know or are they just saying that because it is suposed to be for research only????

I dont know what to say, can someone give me a good reply

Thanks,

Piason..........