Reply With QuoteTweetwhat about the hole in the rubber stopper; can bacteria get in there or somthin?
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home brewers: gear ruined?
Tweetmy bud had a jug of oil he needed to sterilize...got the .2 Whatman.while filtering into a 50ml jug, the pin slipped out a couple times, he put it back in and kept going...when the first 25cc's was done, he left the filter in the jug and refilled the other 25cc then reattached and began filtering again..pin slipped out again a couple times....so when he finished, he baked at 250 for 20 min. w/ a 22g pin to vent...after 20 min...the plastic from the pin is melted, the pin is still there of course...so he cools it off and theres some baked on plastic residue and a tiny hole where the pin was..so the rubber has a tiny hole in it exposing it to air...
he says fine, as long as it was baked and filtered..i say no way cause the hole in the top...is this normal?
Re: home brewers: gear ruined?
Tweetwhat about the hole in the rubber stopper; can bacteria get in there or somthin?
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Platinum
Re: home brewers: gear ruined?
Tweetare you saying there is a hole in the rubber stopper too big to seal itself?...if so, he should have put a new stopper on the vial.
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Re: home brewers: gear ruined?
Tweetyah, there's a hole u can look down into and c the oil, and also the oil will slowly come out if the vial is held upside down...he put some in other vials but im not sure if this is an issue or not
VET
Re: home brewers: gear ruined?
Tweetget a new stopper
Re: home brewers: gear ruined?
Tweetshould it be refiltered and baked again since there was a hole in the stopper?
Platinum
Re: home brewers: gear ruined?
Tweeti think it will be fine if you put it in a new bottle or new stopper in that one!Originally Posted by bodyodyo1
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Re: home brewers: gear ruined?
Tweetwhy bake it? doesn't do anything. It would need to reach over 800 degrees to kill everything. just get new vials and you should be fine but if you want refilter.
Re: home brewers: gear ruined?
Tweetit is NEVER a good idea to bake your gear for the purpose of sterilization. BA and a filter are all you need. i would refilter into a new sterile vial.
Established Resident
Re: home brewers: gear ruined?
TweetTransfer it to a new vial. The filter removes the bacteria, as 0.2um is smaller than bacteria the baking step isn't really necessary.
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